Proliferation in the Alzheimer Hippocampus Is due to Microglia, Not Astroglia, and Occurs at Sites of Amyloid Deposition
Figure 1
GFAP and PCNA expression in the human hippocampus (a, b) Immunolabeled GFAP+ (red) and PCNA+ (blue) cells did not colocalize in the SGZ or other areas of the hippocampus. (c-d) PCNA+ cells (arrows) were observed in the presence of hypertrophic astrocytes with processes infiltrating Aβ plaques (black). (e) A low-magnification image representative of GFAP and Aβ plaques distributed through out the CA areas, with heavy immunostaining in the stratum moleculare and stratum lacunosum. (f) PCNA+ cells were also found in glia-rich regions in the absence of Aβ plaques and in blood vessels (g, h) outlined by astrocytic endfeet. (i) GFAP+ cells were found at higher density in the CA1/2 region in the AD versus control subjects (one-way ANOVA ). (j) PCNA+ cell density was not significantly different between patient cohorts. Scale bars: left panels (a, c, g) = 50 μm, panel (e) = 500 μm. Right panels (b, d, h) = 20 μm, panel (f) = 50 μm.
