Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats

Figure 1

Effects of t-BOOH and NaOCl on mechanical thresholds in normal rats. (a) Intrathecal administration of t-BOOH (11 or 28 μmol) or NaOCl (134 μmol) temporarily decreased the mechanical threshold in a dose-dependent manner, compared to the mechanical threshold observed with administration of saline. Asterisks indicate values that are significantly different from those for the saline group as determined by a two-way repeated-measures analysis of variance with one repeated factor followed by the Duncan post hoc test (). (b) Mechanical thresholds of hind paws after intrathecal administration of t-BOOH (11 μmol, ; 28 μmol, ) or NaOCl (134 μmol, ), compared with the mechanical threshold observed with saline, 30 min after injection. Asterisks indicate values that are significantly different from that for the saline group as determined by the Kruskal-Wallis analysis of variance by ranks followed by the Dunn test (). The data are mean with standard errors of the mean. VF, von Frey filament sizes.

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