Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats

Figure 3

Effects of t-BOOH on membrane potential and EPSP in a patch clamp recording of SG neurons. (a) Original amplitude traces of EPSP in an SG neuron show that t-BOOH (2 mM, 7 min) increased the amplitude and frequency of EPSPs. The lower traces show EPSP at an expanded time scale. Normalized cumulative distribution analysis of EPSP amplitude and frequency (lower panels) showed that t-BOOH caused a significant shift toward higher frequency (left) and amplitude (right) in the neuron. (b) Average EPSP frequency (left) and amplitude (right). After application of t-BOOH, both the frequency and the amplitude increased significantly (, asterisks) from pre-t-BOOH levels as determined by a paired -test. The data are mean with standard errors of the mean.

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