Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats

Figure 4

Effects of t-BOOH on the excitability of SG neurons in spinal cord slices. (a) Recordings of action potentials generated in an AFN. Action potentials were evoked by direct intracellular injections of current pulses (25 or 50 pA). (b) Changes in the frequency of action potential in a TFN. After t-BOOH treatment, the frequency of action potentials increased, compared to that observed with the vehicle treatment. (c) Average action potential firing rate measured by step current pulses after t-BOOH treatment in AFNs (). The data are mean with standard errors of the mean. (d) Average action potential firing rate measured by step current pulses after t-BOOH treatment in TFNs (). Asterisks indicate values that are significantly different from those for the vehicle control as determined by a paired t-test ().

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