Deficient Purposeful Use of Forepaws in Female Mice Modelling Rett Syndrome

Figure 5

Brain metabolic profile. (a) Examples of in vivo sagittal T2-weighted spin-echo MR images (TR/TEeff = 3000/60 ms, slice thickness 0.8 mm, ns = 4, 13 slices, FOV = 25 × 25 mm², and matrix 256 × 256). (b) Example of in vivo  ¹H MR spectra (PRESS, TR/TE = 4000/23 ms, NS = 256) acquired from a voxel positioned in the striatum (indicated by the white rectangles in (a)) of a wild-type (wt) and MeCP2-308 female mice (Het). (c) and (d) Metabolite levels obtained from in vivo  ¹H spectra in the hippocampus (c) and the striatum (d), obtained by using a quantitative protocol (which uses water as internal standard) and LCModel fitting program. Metabolite assignments: Cr, creatine; Gln, glutamine; Glu, glutamate; GPC, glycerophosphocholine; Ins, myo-inositol; NAA, nacetylaspartate; NAAG, n-acetylaspartylglutamate; PCho, phosphocholine; PCr, phosphocreatine; Tau, taurine. Data are expressed as mean ± SEM. .

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