PKA Inhibitor H89 (N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulfonamide) Attenuates Synaptic Dysfunction and Neuronal Cell Death following Ischemic Injury

Figure 4

The measurement of MAP2 mRNA level in Neuro2A cells after PKA inhibitor treatment. MAP2 mRNA levels were measured by using RT-PCR. The H89 group showed higher mRNA levels of MAP2 compared to the hypoxia reperfusion injury group. Data were expressed as mean ± S.E.M, and each experiment included 3 repeats per condition. GAPDH was used as a control. Differences were considered significant at and . Normal: the normal control group, H/R: 4 hr hypoxia and 18 hr reperfusion injury group, and H89: 2 hr PKA inhibitor H89 treatment group before 4 hr hypoxia and 18 hr reperfusion injury.