Activation of the Mammalian Target of Rapamycin in the Rostral Ventromedial Medulla Contributes to the Maintenance of Nerve Injury-Induced Neuropathic Pain in Rat

Table 1

Antisera used in each group.

Group	Primary antisera	Secondary antisera
p-mTOR/NeuN	Rabbit anti-p-mTOR; (1 : 200, Cell Signaling Technology, Danvers, MA, USA); Mouse anti-NeuN (1 : 500, Millipore, Temecula, CA, USA)	Alexa 594 donkey anti-rabbit; (1 : 500, Invitrogen, Camarillo, CA, USA); Alexa 488 donkey anti-mouse (1 : 500, Invitrogen)
p-mTOR/FG	Rabbit anti-p-mTOR (1 : 200, Cell Signaling Technology); Guinea pig anti-FG; (1 : 200, Protos Biotech, New York, NY, USA)	Alexa 594 donkey anti-rabbit (1 : 500, Invitrogen); Alexa 488 donkey anti-guinea pig (1 : 500, Invitrogen)
p-mTOR/5-HT	Rabbit anti-p-mTOR (1 : 200, Cell Signaling Technology); Goat anti-5-HT (1 : 500, Immunostar, Hudson, WI, USA)	Alexa 594 donkey anti-rabbit (1 : 500, Invitrogen); Alexa 488 donkey anti-goat (1 : 500, Invitrogen)