α7 Nicotinic Receptor Promotes the Neuroprotective Functions of Astrocytes against Oxaliplatin Neurotoxicity

Figure 3

Effect of PNU in neuron monoculture. (a) Cell viability. Neurons (10⁴ cells/well) were incubated with oxaliplatin (0.3–100 μM) in the absence or presence of 10 μM PNU for 48 h. Viability was quantified by MTT assay. Values are expressed in percentage of control absorbance as mean ± S.E.M. of 6 experiments. Control condition absorbance was fixed to 100%. versus control. (b) Caspase-3 activity. Neurons (7 10⁵ cells/well) were incubated with 1 μM oxaliplatin in the absence or presence of 10 μM PNU for 48 h. The enzymatic activity was measured by a fluorescent assay. Values are expressed as percent of control caspase-3 activity arbitrarily set as 100%. One-way ANOVA was performed followed by a Bonferroni significant difference procedure. Bars represent mean ± S.E.M. of 3 experiments. versus control.

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