Dehydration-Induced Anorexia Reduces Astrocyte Density in the Rat Corpus Callosum

Figure 1

The rat corpus callosum (CC). (a) Sagittal sections from young female rat brains were immunostained for GFAP, and nuclei were labeled with DAPI. The CC was divided into three regions: genu (G), body (B), and splenium (S) to determine glial cell density. Other brain regions are indicated for anatomical reference: caudate-putamen (CPu), hippocampus (Hip), and lateral ventricle (LV). Arrows show anatomical orientation: dorsal (D), ventral (V), anterior (A), and posterior (P). (b) Higher magnification of the caudal body of the rat CC; notice that the lateral ventricle (LV) contains the choroid plexus (ChP) in close interaction with GFAP-positive cells from the CC. (c) Higher magnification of the square in (b) to highlight fibrous astrocytes from the body of the CC; notice that nuclei stained by DAPI show a radial orientation, and astrocytes align accordingly (arrowheads). Scale bars: 200 μm (a), 100 μm (b), and 50 μm.

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