Alterations of the Ceramide Metabolism in the Peri-Infarct Cortex Are Independent of the Sphingomyelinase Pathway and Not Influenced by the Acid Sphingomyelinase Inhibitor Fluoxetine

Figure 1

Ceramide, its precursors, and its derivate levels are altered in the peri-infarct cortex after photothrombotic stroke. (a) Total ceramide levels, (b) dihydroceramide levels, (c) sphinganine levels, (d) sphingosine levels, (e) glucosylceramide levels, and (f) lactosylceramide levels. Sphingolipids were measured at the indicated time points by tandem mass spectrometry. Differences between sham and PT group were analyzed using Student’s unpaired two-tailed -test. Data are presented as means ± SD; sham values are indicated by dotted line (for individual sham-SD’s, see Section 3); values are not significantly different compared to sham if not marked otherwise; ; ; n.s., nonsignificant; = 8–10/group.

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