Localization of Presynaptic Plasticity Mechanisms Enables Functional Independence of Synaptic and Ectopic Transmission in the Cerebellum

Figure 8

Effect of activation of NMDA receptors on Purkinje neuron responses. (a) Representative whole-cell recordings of EPSCs generated by paired pulse stimulation (100 ms interval) of parallel fibres in transverse cerebellar slices at 0.033 Hz before (first panel), after 10 minutes incubation with 30 μM NMDA and 10 μM glycine (second panel), and after 30 minutes of washout (third panel). (b) Time course of 30 μM NMDA and 10 μM glycine effect on amplitude of the first pulse in each pair, with NMDA and glycine incubation at the time indicated by the bar. Data are mean ± s.e.m. from 5 cells. , one-sample -test. (c) Time course of 30 μM NMDA and 10 μM glycine effect on paired pulse ratio, with NMDA and glycine incubation at the time indicated by the bar. Data are mean ± s.e.m. from 5 cells.

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