Research Article

Hunting Increases Phosphorylation of Calcium/Calmodulin-Dependent Protein Kinase Type II in Adult Barn Owls

Figure 8

General success of immunohistochemistry in adult tissue. (a) Immunostaining for CaMKII (green), tyrosine hydroxylase (red), DARPP-32 (blue), and overlay (bottom right). (b) Ultrasensitive detection of αCaMKII with primary antibody dilution at 1 : 5,000 (left), 1 : 20,000 (middle), and 1 : 100,000 (right). Red channel is EF1α at 1 : 1,000 detected with standard procedure.
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