CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation

Figure 3

CRMP2 and CRMP4 form complexes in growth cones. (a) Growth cones lysates from rat brain were subjected to glutathione S-transferase- (GST-) pulldown assays using GST-CRMP2 or GST-CRMP4. The retrieved sediments were subjected to western blot analysis using the CRMP4 or CRMP2 antibodies. The GAPDH antibody was used to show equal loading. (b) Growth cones lysates from rat brain were subjected to coimmunoprecipitation (Co-IP) assays with rabbit CRMP2 and CRMP4 antibodies, and the resulting sediments were subjected to western blot analysis with mouse CRMP4 or CRMP2 antibodies. (c) Rabbit anti-CRMP2 and mouse anti-CRMP4 antibodies were used to detect endogenous proteins in the growth cones of hippocampal neurons. Scale bar: 10 μm.

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