Chondroitin 6-Sulfation Regulates Perineuronal Net Formation by Controlling the Stability of Aggrecan

Figure 2

Sequential extraction of PNN components from adult mouse brain. Six-month-old adult WT and C6ST-1 TG mouse brain was sequentially extracted as described in Section 2. The same protein amount of the extracts was analyzed and intensities of the indicated bands were determined as relative values to WT mice for each extract. (a) The total level of aggrecan in C6ST-1 TG mice was significantly decreased compared with WT mice. Aggrecan levels in both the PBS extract and 6 M urea extract slightly reduced in C6ST-1 TG mice although this did not reach statistically significant difference. The levels of neurocan (b), phosphacan (c), brevican (d), tenascin-R (e), and Crtl1 (f) were not different between WT and C6ST-1 TG mice. Actin was detected as loading control. Asterisks denote significant differences (, Student’s t-test) between WT and C6ST-1 TG mice. Error bars represent SEM; for each genotype.

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