Chondroitin 6-Sulfation Regulates Perineuronal Net Formation by Controlling the Stability of Aggrecan

Figure 3

Effects of overexpression of C6ST-1 on the repeating disaccharide region and linkage region of CS chains. (a) Epitopes recognized by antibodies CS56, Δ6S, and Δ4S. 6S and 4S represent 6-sulfation and 4-sulfation, respectively. (b) C6ST-1 TG mice showed a delayed decrease in CS56-reactivity as compared with WT in the developing brain. Δ6S (c) and Δ4S (d) antibodies recognized distinct subsets of CSPGs. During early postnatal development, C6ST-1 TG mice exhibited greater Δ6S-reactivity than WT mice, whereas Δ4S-reactivity was not different between the two groups. (e) Distinct localization of Δ6S- and Δ4S-reactive CSPGs in 6-month-old adult cerebral cortex of WT mice. Scale bar, 100 μm.

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