Perspectives of TRPV1 Function on the Neurogenesis and Neural Plasticity

Figure 2

TRPV1 detected by immunofluorescence. (a) Methodology proposed for detection of TRPV1 by immunofluorescence. Using two antibodies against different epitopes of the channel allows corroborating the expression of the channel. In this case, we showed an antibody against the C-terminal and another against the N-terminal. As both antibodies bind to intracellular epitopes, it is advisable to use as internal control of the technique a sample without permeabilization of the plasma membrane, which prevents the entry of the antibody into the cell. One added strategy to improve signal sensitivity was the use of a blocking peptide, in this case, for the C-terminal or N-terminal. The competition of the blocking peptide with the epitope of the channel should diminish the intensity of the signal indicating the specificity of the technique. (b) Detection of TRPV1 in heterologous expression system using antibodies against the N-terminal and C-terminal of TRPV1. (c) Detection of TRPV1 in primate prefrontal cortex, using an antibody against the N-terminal and another against the C-terminal.

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