Perspectives of TRPV1 Function on the Neurogenesis and Neural Plasticity

Figure 3

Expression of TRPV1 in neuronal differentiation process derived from LYON-ES. Determination of expression of TRPV1 by immunofluorescence staining for undifferentiated monkey ESCs stably expressing Tau-GFP (TAU-GFP LYON-ES1 line, a–d), NSCs (NS, e–h), neurons (i–l), and glial cells (m–p) derived from TAU-GFP-LYON-ES1 cells. At each stage, we performed immunofluorescence with antibodies against Oct4 (c) to identify LYON-ESCs, Pax6 for NSCs (g), β-III-tubulin for neurons (k), glial fibrillary acid protein (GFAP) for glial cells (o), and TRPV1 (d, h, l, p). Each experiment was accompanied by nuclear staining with DAPI (a, e, i, m), all the cells having a GFP fused to the microtubule-associated protein tau (b, f, j, n). The magnification of images was 20x for LYON-ESCs and neurons, and 40x for NSCs and glia cells.