Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain

Figure 7

Olig2 and Otx2 expression was not suppressed by miR-344b and miR-344c. (a) Transfection efficiency of miR-344b and miR-344c expression vectors that contained eGFP reporter gene at 48 h. (b) Stem-loop RT-qPCR expression profiles of miR-344b and miR-344c in transfected HEK293 cells. Graph bars of each transfection period were represented as mean ± standard error of mean (SEM). Log₂ expression profiles of miR-344b and miR-344c were normalized to Hmbs. Asterisks denote the statistical significance at () based on one-way analysis of variance. The luciferase assay was performed to validate the predicted target gene was a direct target of miR-344b (c) and miR-344c (d) 24, 36, 48, and 60 h after transfection. Group 1 = negative control luciferase vector + miRNA expression vector; Group 2 = target gene luciferase vector + miR-scramble; Group 3 = target gene luciferase vector; Group 4 = negative control luciferase vector; Group 5 = target gene luciferase vector + miRNA expression vector.

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