Seizure-Induced Regulations of Amyloid-β, STEP₆₁, and STEP₆₁ Substrates Involved in Hippocampal Synaptic Plasticity

Figure 2

A single ECS but not chronic ECS transiently decreases the level of Tyr¹⁴⁷²-phosphorylated GluN2B in the hippocampus. Immunoblot analysis for the phosphorylation of GluN2B at Tyr¹⁴⁷² (Y¹⁴⁷²) and total GluN2B expression in the hippocampal crude membrane (P2) fraction following a single ECS ( rats per time point) (a) and chronic ECS ( rats per time point) (b). The ratio of the Tyr¹⁴⁷²-phosphorylated GluN2B band intensity over the β-actin band intensity (top graphs) and the ratio of total GluN2B band intensity over the β-actin band intensity (bottom graphs) were calculated per each time point and normalized to that of “no seizure” (NS) sham group. Data shown represent the mean band intensity ± SEM. (a) A single ECS transiently decreases the level of Tyr¹⁴⁷²-phosphorylated GluN2B in the hippocampus at 48 h () and 72 h () following a single ECS. (b) Chronic ECS significantly decreases total GluN2B expression over the time course of 96 h in the hippocampus (, , and ).

(a)

(b)