Seizure-Induced Regulations of Amyloid-β, STEP₆₁, and STEP₆₁ Substrates Involved in Hippocampal Synaptic Plasticity

Figure 3

A single ECS but not chronic ECS increases the level of Tyr⁸⁷⁶-phosphorylated GluA2 in the hippocampus. Immunoblot analysis for phosphorylation of GluA2 at Tyr⁸⁷⁶ (Y⁸⁷⁶) or 3Tyr (3Y: Tyr⁸⁶⁹, Tyr⁸⁷³, and Tyr⁸⁷⁶) and total GluA2 expression in the hippocampal crude membrane (P2) fraction following a single ECS ( rats per time point) (a–c) and chronic ECS ( rats for 72 h time point and rats per all other time points) (d–f). The ratio of the phosphorylated GluA2 band intensity over the β-actin band intensity (a-b, d-e) and the ratio of total GluA2 band intensity over the β-actin band intensity (c, f) were calculated per each time point and normalized to that of “no seizure” (NS) sham group. Data shown represent the mean band intensity ± SEM. (a–c) A single ECS increases the level of Tyr⁸⁷⁶-phosphorylated GluA2 at 96 h time point ((b) ) but does not alter the level of 3Tyr-phosphorylated GluA2 and total GluA2. (d–f) Chronic ECS does not change Tyr-phosphorylation of GluA2 (d-e) and total GluA2 expression (f).

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