Seizure-Induced Regulations of Amyloid-β, STEP₆₁, and STEP₆₁ Substrates Involved in Hippocampal Synaptic Plasticity

Figure 5

A single ECS and chronic ECS increase APP and oligomeric Aβ expression in the hippocampus. Immunoblot analysis of APP and oligomeric Aβ in the hippocampal crude soluble (S2) fraction following a single ECS ( rats per time point) (a) and chronic ECS ( rats per time point) (b). The ratio of the Aβ band intensity over the β-actin band intensity (top graphs) and the ratio of the APP band intensity over the β-actin band intensity (bottom graphs) were calculated per each time point and normalized to that of “no seizure” (NS) sham group. Data shown represent the mean band intensity ± SEM. (a) A single ECS increases Aβ expression at 72 h (, -test) and APP expression at 72–96 h following a single ECS (, ). (b) Chronic ECS increases Aβ expression at 48 h and 96 h, as well as APP expression at 48 h following chronic ECS (, ).

(a)

(b)