Anorexia Reduces GFAP+ Cell Density in the Rat Hippocampus

Figure 1

The rat hippocampus. Coronal brain sections from young female rats showing the hippocampus of control, dehydration-induced anorexia (DIA), and forced food-restricted (FFR) rats. The histological sections from the three experimental groups were immunostained for GFAP (green), and nuclei were labeled with DAPI (blue). The hippocampal regions for this study included the cornus ammonis 1–3 (CA1, CA2, and CA3) and the dentate gyrus (DG). Nuclear and astrocyte densities were estimated in the stratum oriens (s.o.) and stratum radiatum (s.r.) of CA1, CA2, and CA3, while the hilus region () was studied for the DG (squares). Other regions are indicated for anatomical reference: stratum pyramidale (s.p.), granular cell layer (GCL), and subgranular zone (SGZ). Arrows show anatomical orientation: dorsal (D), ventral (V), and lateral (L). Scale bars correspond to 200 μm.