Astaxanthin Protects Primary Hippocampal Neurons against Noxious Effects of Aβ-Oligomers

Figure 4

ATX prevents AβOs-induced RyR2 mRNA downregulation. Hippocampal cultures were preincubated with 0.1 μM ATX (a) or 10 mM of NAC (b) for 1.5 h or 30 minutes, respectively, before incubation for 6 h with 500 nM AβOs. Relative RyR2 mRNA levels were determined with qPCR, normalized to β-actin mRNA levels, and expressed as fold over control. Values represent mean ± SE () from experiments performed in cultures from six different animals; each condition was tested in triplicate. Statistically significant differences among experimental conditions were evaluated by one-way ANOVA followed by Bonferroni’s multiple comparison test ( compared to control; compared to indicated conditions).

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