Postischemic Anhedonia Associated with Neurodegenerative Changes in the Hippocampal Dentate Gyrus of Rats

Figure 12

Analysis of Ki67 immunopositive cells in GCL. (a) Typical images of the slices taken at 30 weeks after MCAO. Scale bar indicates 20 μm. (b) Time course changes of Ki67 immunopositive cells after MCAO in GCL. Symbols used are as follows: open square, sham; closed triangle, ipsilateral MCAO; open triangle, contralateral MCAO. Statistical significance was evaluated with two-way ANOVA followed by Tukey-Kramer test at each time point (; ; ; versus sham). (c) Analysis of the effect of imipramine (IMP) on the number of Ki67 immunopositive cells at 30 weeks after MCAO. Vehicle [IMP(−)] or 20 mg/kg/day of IMP for 29 weeks [IMP(+)] from 1 week after the surgical operation was administered. Statistical significance was evaluated with one-way ANOVA followed by Tukey-Kramer test (; and versus sham [IMP(−)]; versus contralateral MCAO [IMP(−)]; /group). Unpaired -test was performed with sham [IMP(−)] versus [IMP(+)] (, ) or with ipsilateral MCAO [IMP(−)] versus [IMP(+)] (, ).

(a)

(b)

(c)