Postischemic Anhedonia Associated with Neurodegenerative Changes in the Hippocampal Dentate Gyrus of Rats

Figure 5

Analysis of βIII-tubulin immunopositive areas in GCL. (a) Typical images of the slices taken at 30 weeks after MCAO. Scale bar indicates 30 μm. (b) Time course changes of βIII-tubulin immunopositive areas after MCAO in GCL. Symbols used are as follows: open square, sham; closed triangle, ipsilateral MCAO; open triangle, contralateral MCAO. Statistical significance was evaluated with two-way ANOVA followed by Tukey-Kramer test at each time point (; ; ; and versus sham). (c) Analysis of the effect of IMP on βIII-tubulin immunopositive areas at 30 weeks after MCAO. Vehicle [IMP(−)] or 20 mg/kg/day of IMP for 29 weeks [IMP(+)] from 1 week after the surgical operation was administered. Statistical significance was evaluated with one-way ANOVA followed by Tukey-Kramer test (; versus sham [IMP(−)]; /group). Unpaired -test was performed with ipsilateral MCAO [IMP(−)] versus contralateral MCAO [IMP(−)] (, ) or versus ipsilateral MCAO [IMP(+)] (, ).

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