Postischemic Anhedonia Associated with Neurodegenerative Changes in the Hippocampal Dentate Gyrus of Rats

Figure 7

Analysis of the number of cleaved caspase-3 immunopositive cells in ipsilateral GCL and SGZ. (a) Typical images of the slices taken at 30 weeks after MCAO. Blue signal is the nuclei stained with Höchst 33258, while green signal of FITC is from cleaved caspase-3 immunopositive cells. Scale bar indicates 50 μm. (b, c) Time course changes of cleaved caspase-3 immunopositive cells after MCAO in GCL (b) and SGZ (c). Symbols used are as follows: open square, sham; closed triangle, MCAO. Statistical significance was evaluated with two-way ANOVA followed by unpaired -test at each time point (GCL: , , , , , , and ; SGZ: , , , , , , and ; and versus sham). (d, e) Analysis of the effect of IMP (20 mg/kg/day for 29 weeks) on the number of cleaved caspase-3 immunopositive cells at 30 weeks after MCAO in GCL (d) and SGZ (e). Statistical significance was evaluated with one-way ANOVA followed by Tukey-Kramer post hoc test (; ; versus sham [IMP(−)]; versus MCAO [IMP(−)]; /group).

(a)

(b)

(c)

(d)

(e)