Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons

Figure 2

Effects of 30 μM carbachol on the AP firing of RA projection neurons evoked by a depolarizing stimulus of 100 pA amplitude at 500 ms duration. (a) Sample traces showed that carbachol decreased the number of spikes evoked by depolarizing step pulse and induced membrane hyperpolarization. (b) The number of spikes evoked by depolarizing step pulse was significantly decreased in the presence of carbachol (). (c) The evoked AP latency was significantly increased in the presence of carbachol (, the latency is determined from the time between the onset of the stimulus and the first evoked AP for each cell). (d) Membrane potential was significantly hyperpolarized in the presence of carbachol (). means , paired test.