Effect of Maternal Administration of Edible Bird’s Nest on the Learning and Memory Abilities of Suckling Offspring in Mice

Table 1

Details of grouping and oral administration of the test substances.

Group number	Group name	Test substance	Concentration	Period of the treatment	Length of treated time (d)
1	Negative control (CK)	Normal saline	/	Pregnancy and lactation	42
2	Positive pregnancy control (PP)	N-acetylneuraminic acid	0.5 mg/mL	Pregnancy	21
3	Positive lactation control (PL)	N-acetylneuraminic acid	0.5 mg/mL	Lactation	21
4	Positive pregnancy and lactation control (PPL)	N-acetylneuraminic acid	0.5 mg/mL	Pregnancy and lactation	42
5	Pregnancy and EBN low dose (PEL)	EBN homogenate	4.5 g/182 g	Pregnancy	21
6	Pregnancy and EBN middle dose (PEM)	EBN homogenate	6.75 g/182 g	Pregnancy	21
7	Pregnancy and EBN high dose (PEH)	EBN homogenate	9 g/182 g	Pregnancy	21
8	Lactation and EBN low dose (LEL)	EBN homogenate	4.5 g/182 g	Lactation	21
9	Lactation and EBN middle dose (LEM)	EBN homogenate	6.75 g/182 g	Lactation	21
10	Lactation and EBN high dose (LEH)	EBN homogenate	9 g/182 g	Lactation	21
11	Pregnancy and lactation EBN low dose (PLEL)	EBN homogenate	4.5 g/182 g	Pregnancy and lactation	42
12	Pregnancy and lactation EBN middle dose (PLEM)	EBN homogenate	6.75 g/182 g	Pregnancy and lactation	42
13	Pregnancy and lactation EBN high dose (PLEH)	EBN homogenate	9 g/182 g	Pregnancy and lactation	42

All the female mice from each group were treated with 0.5 mL/d N-acetylneuraminic acid or EBN homogenate in their corresponding period. As for the negative control group, mice were treated with saline (0.5 mL/d).