Wnt1 Promotes EAAT2 Expression and Mediates the Protective Effects of Astrocytes on Dopaminergic Cells in Parkinson’s Disease

Figure 2

6-OHDA treatment downregulated the protein level of Wnt1 in U251 cells. (a) U251 cells were treated with vehicle or 6-OHDA (50 μM) for 3, 6, 9, 12, and 24 h, and the protein level of Wnt1 was detected by Western blot with β-actin as an internal control. (b) The relative band intensities of Wnt1 were measured by Quantity One software and normalized to the expression of β-actin in U251 cells. All of the data are presented as the from three independent experiments. Differences among various time points were examined by using ANOVA followed by Tukey-Kramer tests for post hoc multiple comparisons. value = 67.10, , , and .

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