Wnt1 Promotes EAAT2 Expression and Mediates the Protective Effects of Astrocytes on Dopaminergic Cells in Parkinson’s Disease

Figure 5

Effect of coculturing with U251-Wnt1 cells on 6-OHDA-induced damage in SH-SY5Y cells measured by flow cytometry. SH-SY5Y cells were indirectly cocultured with U251 cells, U251-Wnt1 cells, or U251-EV cells and then treated with or without 50 μM 6-OHDA or 100 ng/ml DKK-1 for 24 h, and a combination of annexin V-FITC and PI staining was performed followed by flow cytometry. (a) Representative set of flow cytometric two-parameter dot plots in the corresponding groups. The lower right quadrant, which shows annexin V-FITC-positive and PI-negative cells, represents early apoptotic cells. The upper right quadrant, which shows both annexin V-FITC- and PI-positive cells, represents cells in the end stages of apoptosis and necrosis. (b) The percentage of cells under apoptosis and necrosis. Data are presented as the from 3 independent experiments. Differences among groups were examined by using ANOVA followed by Tukey-Kramer tests for post hoc multiple comparisons. value = 28.59, , , and .

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