Enhanced Glutamatergic Currents at Birth in Shank3 KO Mice

Figure 2

Spontaneous glutamatergic network activity is already enhanced in CA3 pyramidal neurons of Shank3^-/- mice at birth. (a) Example of traces of sEPSCs recorded in whole-cell patch clamp at -75 mV in WT (in black) and Shank3^-/- (in red) mice at P0. (b, c) Bar graphs show the average frequency (b) and amplitude (c) of sEPSCs at P0. (d, e) Cumulative probability distributions of interevent interval (iei; d) and amplitude (e) in WT and Shank3^-/- mice at P0. (f) Representative traces of sEPSCs recorded in whole-cell patch clamp at -75 mV in WT (in black) and Shank3^-/- (in red) mice at P15. (g, h) Bar graphs show the average frequency (g) and amplitude (h) of sEPSCs at P15. (i, j) Cumulative probability distributions of iei (i) and amplitude (j) in WT and Shank3^-/- mice at P15. Data are presented as , , . (b–e) for WT and for Shank3^-/- mice. (g–j) for WT and for Shank3^-/- mice.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)