Administration of Recombinant Heat Shock Protein 70 Delays Peripheral Muscle Denervation in the SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis
Figure 6
Treatment with rhHsp70 has no effect on ER stress in the spinal cord as determined by BiP expression. (a) Protein extracts were collected from individual lumbar spinal cords and subjected to electrophoresis on a 12% SDS PAGE gel. Proteins were transferred to Immobilon-P membrane, blocked in TBS-T, incubated with a BiP antibody (Cell Signaling) and a donkey anti-rabbit secondary antibody (Jackson Immuno), reacted in ECL, and exposed to X-ray film. (b) Relative levels of BiP were determined using densitometry ( blots for each spinal cord). There was only a slight increasing trend in relative levels of BiP when comparing SOD1-untreated to SOD1-treated and WT mice. Statistical significance was determined using a two-way ANOVA followed by Tukey-Kramer post hoc test.
