The Clinical and Prognostic Significance of Activated AKT-mTOR Pathway in Human Astrocytomas
Table 4
Summary of reports investigating the role of phosphorylated 4E-BP1 in astrocytomas.
Report (number of cases and grades)
Antibody used
Immunostaining percentage
Correlations with clinicopathological features
Other correlations
Survival analysis
Korkolopoulou et al. [30] (111 patients: 25 grade II, 15 III, and 71 IV)
Rabbit polyclonal anti-p-4E-BP1/2/3 (Ser36) ab (SCB), diluted 1 : 50 for IHC and 1 : 200 for WB
82.4% (61/74) nuclear
p-4E-BP1 with tumor grade
p-4E-BP1 with (i) nuclear p-AKT (ii) cytoplasmic p-ERK (iii) p-mTOR (iv) VEGF
(i) p-4E-BP1 adversely affected survival in the entire cohort and marginally in glioblastomas (ii) Multivariate analysis: p-4E-BP1 as an independent predictor of survival in the entire cohort as well as in glioblastomas
Ermoian et al. [36] (71 patients: 28 grade II, 17 III, and 26 IV