Gene Regulation Systems for Gene Therapy Applications in the Central Nervous System
Figure 1
Mechanics of tetracycline regulated systems. A constitutively active promoter drives expression of tetracycline transactivators (tTAs) or reverse tetracycline transactivators (rtTAs). (a) Tet-Off system. tTAs are able to bind to the tet-operator sequence (tetO) in the absence, but not in the presence of doxycycline (dox) to drive transgene expression. (b) Tet-On system. rtTAs are able to bind to the TRE in the presence, but not in the absence of dox to drive transgene expression. tetR: tetracycline repressor; VP16: viral protein 16; CMV: cytomegalovirus.
