Gene Regulation Systems for Gene Therapy Applications in the Central Nervous System

Figure 4

Schematic of the RU486 regulation system. A constitutively active promoter drives expression of a fusion protein consisting of a VP16 activation domain, a Gal4 DNA-binding domain (DBD) and a progesterone ligand-binding domain (Prog LBD). Binding of RU486 to the Prog LBD enables dimerization and binding to 4x Gal 4-binding sites upstream of a TATA box driving transgene expression [28]. Poly A, poly adenylation signal.