Research Article

Short and Long-Term Analysis and Comparison of Neurodegeneration and Inflammatory Cell Response in the Ipsilateral and Contralateral Hemisphere of the Neonatal Mouse Brain after Hypoxia/Ischemia

Figure 12

Leukocyte infiltration is monitored by analysing neutrophils (Ly6B2 staining) and T-lymphocytes (CD3 staining). Ly6B2 staining in the control animals at P7 shows a few neutrophils inside the blood vessels in the hippocampal fissure (a), in DG (b), and in the CA (c) regions. In the hippocampus the maximum density of cells is observed at 7 days after hypoxia especially in the hippocampal fissure (d); CA3 (e) and CA1 (f). In caudate-putamen an increase is observed at 7 days after hypoxia (h). In the ventricles (g) and the neocortex (i) the maximum quantity of neutrophils is observed at 3 hours after hypoxia. No significant difference was observed in the thalamus (j). CD3 staining in the control animals shows the presence of lymphocytes in the meninges (k) and in the ventricles (l). Finally, scattered cells are observed in the neocortex (m). Scale bars: 10 μm. Hf: hippocampal fissure; DG: Dentate gyrus; CA: cornu ammunis.
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