Research Article

Short and Long-Term Analysis and Comparison of Neurodegeneration and Inflammatory Cell Response in the Ipsilateral and Contralateral Hemisphere of the Neonatal Mouse Brain after Hypoxia/Ischemia

Figure 8

GFAP+ area on the hippocampus, corpus callosum, caudate-putamen, neocortex, and thalamus is evaluated from 3 hrs to 100 days after hypoxia in the ipsilateral (IL) and contralateral (CL) hemispheres. Astrogliosis is shown as the percentage of the GFAP+ area (see Section 2 for details). The hippocampus and caudate-putamen are the most affected regions and significant differences between IL and CL hemispheres are found at all time points. The astroglial reactivity in corpus callosum is observed at 12 hrs after hypoxia between IL and CL. Higher astrogliosis is observed in the ipsilateral neocortex at almost all times analyzed compared to CL side. A significant increase in astroglial activation after 72 hrs is seen in the IL thalamus. Significant differences between IL and CL hemisphere are shown using unpaired t tests, with Welch’s correction if suitable (* , ** , *** ). Individual data and mean ± S.E.M, are represented to show the dispersion in each group.
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