Short and Long-Term Analysis and Comparison of Neurodegeneration and Inflammatory Cell Response in the Ipsilateral and Contralateral Hemisphere of the Neonatal Mouse Brain after Hypoxia/Ischemia

Figure 9

Iba-1 immunostaining showing age-matched controls and H/I effects on the hippocampus and corpus callosum. Developmental changes in microglia are observed in control animals (a–c), showing a progressive change from mainly primitive ramified and amoeboid cells (a) to a resting morphology at P14 (b) and P21 (c). Activated microglia increases in the contralateral (CL) (d) and ipsilateral (IL) hemisphere at 3 hrs (g). At the CL side, Iba-1 shows the maximum labeling at 24 hrs (e) in the hippocampal fissure, returning to control level at 72 hrs (f). In the IL side, higher proportion of amoeboid cells is seen from 24 hrs (h) to 72 hrs (j). At 7 days after hypoxia (n), a reduction on the level of microglia morphologically activated is observed, returning to the basal level at 14 days (o). Resting morphology is observed at 30 (p) and 100 days (q). Detailed morphology of Iba-1+ cells in the cc (k), hf (l), and CA1 (m) observed after 48 hrs and 14 days after H/I (r, s and t, resp.) are shown. Scale bars: in low magnifications: (a) to (j) and (n) to (q) = 100 μm; scale bar in (k, l, m r, s, and t) = 20 μm. CA1: cornu ammonis 1; hf: hippocampal fissure; CC: corpus callosum.