G Protein-Coupled Estrogen Receptor-Selective Ligands Modulate Endometrial Tumor Growth

Figure 2

Subcellular localization of GPER expression in primary mouse uterine epithelial cells. Cells were stained with either an antibody targeted against the carboxy terminus (C-Term) or amino terminus (N-Term) of GPER under either permeabilizing or nonpermeabilizing conditions. As the amino terminus of GPCRs would be extracellular, any plasma membrane-localized receptor should be stained by the amino terminus-targeted antibody under nonpermeabilizing conditions. GPER is stained green; 𝛽-catenin is stained red as a plasma membrane marker. Arrow indicates nuclear membrane.