Protein Kinase Cα Modulates Estrogen-Receptor-Dependent Transcription and Proliferation in Endometrial Cancer Cells
Figure 4
Reconstitution of PKCα regulated, ER-dependent transcription in HEC-50 cells. Cells were transiently transfected with 0.5 μg pHEGO (ERα) and (a) 0.5 μg pEREluc or (b) 0.5 μg pPS2luc and 0.3 μg pCMVβ in the presence or absence of 0.5 μg pmyrPKCα or vector control (pCDNA3). Cells were treated with ±100 nM estradiol (E2), as indicated. Promoter activity was determined as in Figure 2. Data are mean ± s.e.m of 6 experiments conducted in triplicate.
