Research Article

Protein Kinase Cα Modulates Estrogen-Receptor-Dependent Transcription and Proliferation in Endometrial Cancer Cells

Figure 6

Knockdown of PKCα reduces ER-dependent gene expression. Ishikawa cells were stably transduced with shRNAs targeting PKCα or luciferase (luc). RNA was isolated and analyzed by real time reverse transcription PCR, using primers specific for the indicated gene, as described in Section 2. ΔCt values were calculated relative to a control gene (rp13) and relative levels expressed as . Data are mean ± s.e.m .
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