Table 1: (a) Benign hysterectomy specimens: RNA and DNA yield for FF pipelle samples preserved in MEM, FF curettage samples preserved either in MEM or in RNAlater, and FFPE hysterectomy samples. (b) Archived FFPE Specimens: RNA and DNA yield and year of collection for normal and involved tissue samples, for complex hyperplasia and atypical hyperplasia. (c) Archived FFPE Specimens: RNA and DNA yield and year of collection for endometrial carcinomas and normal myometrium.
(a)

Study ID Specimen collection date Age Menopause
status
Pipelle, MEMCurettage, MEMCurettage,
RNAlater
Hysterectomy, FFPE
DNA
yield
( g)
Weight of RNA tissue
(mg)
RNA
yield
( g)
DNA
yield
( g)
Weight of RNA tissue
(mg)
RNA yield
( g)
DNA
yield
( g)
Weight of
RNA tissue
(mg)
RNA yield
( g)

1200946Pre24316226.62650.1352013.4
2200950Perin/a*29238.52932.828.12116.8
3200970Post101.9n/a*n/a*23.4 217.8
4200945Pre20.81925.440.33011.8212418.2
5200951Pre37.73120.917.5 252.7
6200952Peri693227.935.81825.1 213.2
7200947Peri25.3274448.7203449214.4
8200948Pre46.32344.617.22642.2137.53726
Average yield46.4 27.4 35.4 27.3 24.8 32.7 54.1 23.8 11.6

Not enough tissue remaining for nucleic acid extraction.
Not enough yield to use tissue on downstream applications; <1.2  g. These are not included in the average yield.
No tissue available.
Too little tissue to weigh accurately.
(b)

Complex hyperplasia,
Study ID Specimen collection date Age Menopause statusInvolvedUninvolved
Weight of RNA tissue (mg)RNA yield ( g)Weight of RNA tissue (mg)RNA yield ( g)

9200065Post1612.61124.9
10200153Post2316.12818.3
11200338Pre2720.927
12200538Pre361.63214
13200855Post2448.52519.1
14200862Post253.1175.7
Average yield25.2 17.1 23.3 16.4
15200132Pre2110.82818.6
16200354Pre298.22718.2
17200369Post2417.82612.1
18200543Peri279.9269.7
19200554Pre/Peri258256.9
20200872Post2911.82621.4
Average yield25.8 11.1 26.3 14.5

(c)

Endometrial carcinoma,
Study ID Specimen collection date Age Menopause statusInvolvedUninvolved
DNA
yield
( g)
Weight of RNA
tissue
(mg)
RNA
yield
( g)
DNA
yield
( g)
Weight of RNA
tissue
(mg)
RNA
yield
(ng/ul)

21199970Post14.7163040.22343.1
22199984Post89.73236.418.3246.8
23199980Post8.72620.936.82813.7
24200477Post12.52529.651.3296.1
25200470Post33.23014.757.33411
26200455Post83.042127.4
27200781Post40.92319.4
28200759Post17.383527.139.4286.4
29200763Post9.63111.1
30200956Peri81.52432.135.22410.1
31200944Pre49.23218.1
32200952Post44.63239.7
Average yield40.4 27.3 25.5 39.8 27.1 13.9

Not enough tissue remaining for nucleic acid extraction.
Not enough yield to use tissue on downstream applications; <1.2  g. These are not included in the average yield.
No tissue available.
Too little tissue to weigh accurately.