Melatonin Ameliorates Ovarian Hyperstimulation Syndrome (OHSS) through SESN2 Regulated Antiapoptosis

Figure 2

The oxidative stress and SESN2 levels in HUVECs and KGNs induced by different concentrations of angiotensin II or H₂O₂. (A) (a–c) the representative pictures of controls, 1 μM, 2 μM angiotensin II treated HUVECs dyed with DCFH-DA, respectively; (d–f) the representative pictures of controls, 0.1 mM, 0.2 mM H₂O₂ treated KGNs dyed with DCFH-DA, respectively; (B) the comparison of mean oxidative fluorescence intensity in HUVECs. The mean of the results in controls was assigned an arbitrary value of 1.0 and the results (mean ± SD) of other cells are expressed as the relative intensity; (C) the comparison of mean oxidative fluorescence intensity in KGNs. The mean of the results in controls was assigned an arbitrary value of 1.0 and the results (mean ± SD) of other cells are expressed as the relative intensity. Bar: 50 μm; (D) the representative picture of Western blotting of SESN2 and GAPDH in controls, 1 μM, 2 μM angiotensin II treated HUVECs; (E) the representative picture of Western blotting of SESN2 and GAPDH in controls, 0.1 mM, 0.2 mM H₂O₂ treated KGNs; (F) the comparison of SESN2/GAPDH ratio after being incubated with angiotensin II for 24 h; (G) the comparison of SESN2/GAPDH ratio after incubation with H₂O₂ for 12 h. The results are expressed as mean ± SD. ; ; . Ctrl: control.