Role of Oxidative Stress in Transformation Induced by Metal
Mixture
Figure 1
Scheme of two-phase transformation protocol, Balb/c 3T3 cells. Initiation phase, day 1 to 7. On day 1, subconfluent cell culture was treated with MNNG 0.5 g/mL (positive initiator) or metal mixture (2 M NaAsO2, 2 M CdCl2, and 5 M Pb(C2H3O2)2·3H2O) as initiator stimuli during 4 h and reseeded in DMEM medium supplemented with 10% SFB. On day 4 medium was changed. Promotion phase begins on day 7 and ends on day 25. In promotion phase, cells were cultured in DMEM medium supplemented with 2% SFB and 1% ITS-A. On days 7, 11, and 14 cells were treated with TPA 0.1 g/mL (positive promoter) or metal mixture (2 M NaAsO2, 2 M CdCl2 and 5 M Pb(C2H3O2)2·3H2O) as promoter stimuli. Meanwhile, on days 9, 16, 18, and 21 medium changes were done. Sampling days across transformation protocol are represented by “*”; in these days samples were taken before changing media or applying treatment.
