A Sphingosine Kinase Form 2 Knockout Sensitizes Mouse Myocardium to Ischemia/Reoxygenation Injury and Diminishes Responsiveness to Ischemic Preconditioning
Figure 2
Sphingosine kinase activity (SKase) in WT and KO hearts. Hearts from WT and KO hearts were flushed free of blood and then subfractionated into cytosolic and particulate fraction as described in Section 4. The fractions were assayed for SKase activity which is expressed as pmoles per min per mg fraction protein and reveal reduced activity in the KO fractions ( versus WT, ).
