A Sphingosine Kinase Form 2 Knockout Sensitizes Mouse Myocardium to Ischemia/Reoxygenation Injury and Diminishes Responsiveness to Ischemic Preconditioning

Figure 3

Gel filtration profile of cytosolic SKase Activity from WT and KO hearts. The cytosolic fraction isolated from pooled mouse hearts from WT (-●-) and KO (-∘-) hearts were chromatographed on a Sephacryl S200 gel filtration column. The eluted fractions were assayed for SphK activity as described in Section 4, and activity is expressed as cpm of S1P formed per min per 0.1 mL of fraction. The profile is representative of 3 separate experiments and reveal that SphK2 activity is not present in the cytosols from the SphK 2 KO hearts.