145418.fig.001
Figure 1: Total protein content of C2C12 myotubes during cell differentiation. When the C2C12 skeletal muscle cells were differentiated into myotubes, the absorbance of each well (OD) was measured by standard curve with a microplate reader at 450 nm wavelength, and then the protein content was calculated through the standard curve. * , significant difference compared with the first day, # , significant difference compared with the previous day.