Table 1: ROS generation during different electrical stimulation times.

Mean ± SDMean ± SD

Control ES75
ES45 ES90
ES60 ES120

The optical density (OD) values were measured using a fluorescent spectrofluorometer after ES. The peak value of luminescence as a function of time was taken after subtracting the value of the blank signal (luminescence of the illuminated medium).
, significant difference compared with the control group,
, significant difference compared with the 90 min ES group.