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Figure 5: Zinc induces ROS generation and the Pi infusion attenuates ROS production following treatment of astrocytes with zinc. Astrocytes were preloaded with DCF-DA for 30 min and washed with PBS. They were then pre-incubated for 2 h with various concentrations of Pi infusion. Then, ZnCl2 (50 μM) was added and the resulting fluorescence signal was measured at the indicated time points. The results represent means ± SEM of two experiments ( ). compared to cells treated with zinc alone.