185867.fig.002a
(a)
185867.fig.002b
(b)
Figure 2: Protective activity of the grape pomace extract on DNA strand scission induced by OH and ROO. (a) Extract antioxidant activity against OH. Bluescript-SK+ plasmid DNA was exposed to UV plus H2O2 (lane 2) or to UV plus H2O2 in the presence of 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL and 1600 μg/mL of the extract, respectively (lanes 3–7) or to 1600 μg/mL of the extract alone (lane 8). (b) Extract antioxidant activity against ROO. Bluescript-SK+ plasmid DNA was exposed to ROO alone (lane 2) or to ROO in the presence of 1 μg/mL, 2 μg/mL, 5 μg/mL, 10 μg/mL, 50 μg/mL and 100 μg/mL of the extract, respectively (lanes 3–8). Lane 1 represents Bluescript-SK+ plasmid DNA without any treatment. *Significantly different from the control value ( ). OC: open circular conformation of the plasmid, SC: supercoiled conformation of the plasmid.